Whole-brain ex-vivo quantitative MRI of the cuprizone mouse
نویسندگان
چکیده
11 Myelin is a critical component of the nervous system and a major contributor to contrast in Magnetic Resonance (MR) images. However the precise contribution of myelination to multiple MR modalities is still under debate. The cuprizone mouse is a well established model of demyelination that has been used in several MR studies, but these have often imaged only a single slice and analysed a small region of interest in the corpus callosum. We imaged and analyzed the whole brain of the cuprizone mouse ex-vivo using high-resolution quantitative MR methods (multi-component DESPOT, Diffusion Tensor Imaging and Tensor Based Morphometry) and found changes in multiple regions, including the corpus callosum, cerebellum, thalamus and hippocampus. However the presence of inflammation, confirmed with histology, presents difficulties in isolating the sensitivity and specificity of these MR methods to demyelination using this model. 12
منابع مشابه
Whole-brain ex-vivo quantitative MRI of the cuprizone mouse model
Myelin is a critical component of the nervous system and a major contributor to contrast in Magnetic Resonance (MR) images. However, the precise contribution of myelination to multiple MR modalities is still under debate. The cuprizone mouse is a well-established model of demyelination that has been used in several MR studies, but these have often imaged only a single slice and analysed a small...
متن کاملWhole-brain ex-vivo quantitative MRI of the cuprizone
11 Myelin is a critical component of the nervous system and a major contributor to contrast in Magnetic Resonance (MR) images. However the precise contribution of myelination to multiple MR modalities is still under debate. The cuprizone mouse is a well established model of demyelination that has been used in several MR studies, but these have often imaged only a single slice and analysed a sma...
متن کاملSpatio-Temporal Patterns of Demyelination and Remyelination in the Cuprizone Mouse Model
Cuprizone administration in mice provides a reproducible model of demyelination and spontaneous remyelination, and has been useful in understanding important aspects of human disease, including multiple sclerosis. In this study, we apply high spatial resolution quantitative MRI techniques to establish the spatio-temporal patterns of acute demyelination in C57BL/6 mice after 6 weeks of cuprizone...
متن کاملHistological validation of fast macromolecular proton fraction mapping as a quantitative myelin imaging method in the cuprizone demyelination model
Cuprizone-induced demyelination in mice is a frequently used model in preclinical multiple sclerosis research. A recent quantitative clinically-targeted MRI method, fast macromolecular proton fraction (MPF) mapping demonstrated a promise as a myelin biomarker in human and animal studies with a particular advantage of sensitivity to both white matter (WM) and gray matter (GM) demyelination. This...
متن کاملMonitoring Demyelination in a Cuprizone Mouse Model with Longitudinal and Quantitative MRI Measurements
Introduction Magnetic resonance imaging (MRI) methods capable of quantifying changes due to demyelination can improve both the diagnosis and understanding of white matter diseases such as multiple sclerosis. T2-weighted (T2w) and magnetization transfer images (MTI) were acquired weekly in control (n=4) and cuprizone-fed mice (n=4). Diffusion tensor imaging (DTI), quantitative MTI, high-resoluti...
متن کامل